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Crop Wild Relatives in Aragon, Spain, 2024–2025


Collectors: Rik Lievers¹, Cristina Mallor Giménez²³, Ana Isabel Marí León²

¹ Centre for Genetic Resources, the Netherlands (CGN), Wageningen University & Research

² Banco de Germoplasma Hortícola, Centro de Investigación y Tecnología Agroalimentaria de Aragón (BGHZ-CITA), Zaragoza

³ Instituto Agroalimentario de Aragón – IA2, CITA-Universidad de Zaragoza

Introduction

In August 2024 and July 2025, two five-day collecting expeditions for crop wild relatives (CWR) were conducted in the autonomous community of Aragon, northeastern Spain. The expeditions were funded by CGN and carried out together with BGHZ-CITA. The missions were part of a broader collaboration between the two institutes, aimed at the joint collection and ex situ conservation of wild relatives of vegetable crops.

Approval for the collecting activities, including the exchange of collected materials under the SMTA of the ITPGRFA, was obtained from the Dirección General de Medio Natural, Caza y Pesca of the Government of Aragon prior to each mission.

Fieldwork
Cristina Mallor Giménez and Ana Isabel Marí León recording passport data at a Blitum bonus-henricus population in the Moncayo mountains, July 2025.

Collecting activities

The collecting team consisted of three members. The two missions covered complementary parts of Aragon. The 2024 mission (26–30 August) focused on the western Pyrenean valleys and foothills of Huesca province – including the Hecho, Ansó and Fago valleys, the pre-Pyrenean zone (Loarre, Murillo de Gallego, Ayerbe), the Tena valley (Sabiñigo, Biescas, Formigal, up to 1,776 m at Puerto de El Portalet and the Balneario de Panticosa) – and a stop along the Ebro river near Zaragoza.

The 2025 mission (28–31 July) covered a different transect through central and southern Aragon, running from the Ebro lowlands near Borja southwest to Calatayud and Daroca, south through the Sierra de Albarracín and Teruel, into the Gúdar mountains (up to 1,759 m near Valdelinares), and returning north along the eastern foothills toward the Ebro valley. Habitats sampled across both missions included roadsides, field and orchard margins, abandoned fields, rocky slopes, castle walls, riverside paths and mountain meadows. Passport data and GPS coordinates were recorded for each accession, and photographs were taken of the plants in their natural environment.

Map
Collecting sites in Aragon, Spain. Red = 2024 mission; blue = 2025 mission.

Material sampled

We collected 214 accessions of at least 30 species from 12 genera. Some accessions, especially within Allium and Lactuca, require further taxonomic confirmation. A notable find in 2024 was Allium pyrenaicum, a species classified as vulnerable in the Catálogo de Especies Amenazadas en Aragón (Decreto 129/2022); biodiversity data for the two populations were recorded and reported to the competent authority.

The 2025 mission yielded 12 accessions of Lactuca virosa, a species considerably underrepresented in genebank collections relative to its close relative L. serriola, as well as Lactuca perennis and the putatively endemic Allium pardoi.

Botanical name20242025Total
Amaryllidaceae
Allium ampeloprasum189
Allium pardoi?11
Allium pyrenaicum22
Allium schoenoprasum11
Allium sp.549
Allium sphaerocephalon448
Allium vineale11
Amaranthaceae
Beta vulgaris11
Blitum bonus-henricus415
Apiaceae
Daucus carota181533
Foeniculum vulgare22
Pastinaca sativa617
Petroselinum crispum112
Smyrnium olusatrum11
Asparagaceae
Asparagus officinalis11
Asteraceae
Cichorium intybus15924
Cichorium pumilum11
Lactuca perennis11
Lactuca saligna459
Lactuca serriola293160
Lactuca viminea235
Lactuca virosa1212
Brassicaceae
Brassica rapa11
Diplotaxis erucoides156
Eruca vesicaria77
Sinapis sp.112
Fabaceae
Lathyrus sylvestris?11
Phaseolus vulgaris11
Trifolium pratense?11
Total101113214

Conclusion

The expeditions to Aragon successfully collected 214 accessions of crop wild relatives, filling gaps in the Spanish and international genebank collections. Notable additions include the vulnerable Pyrenean endemic Allium pyrenaicum and 12 accessions of Lactuca virosa, a species considerably underrepresented relative to its close relative L. serriola.

In accordance with the agreement between CGN and BGHZ-CITA, CGN is responsible for the multiplication of accessions where seed quantities were insufficient for immediate distribution between the two genebanks. If multiplication is successful, the accessions will be made available to potential users.

Peracense
Lactuca virosa growing at the base of the castle walls of Peracense, July 2025.